stock solution Search Results


genotyping pcr service  (Transnetyx)
99
Transnetyx genotyping pcr service
Genotyping Pcr Service, supplied by Transnetyx, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genotyping pcr service/product/Transnetyx
Average 99 stars, based on 1 article reviews
genotyping pcr service - by Bioz Stars, 2026-02
99/100 stars
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methylcellulose mc stock solution  (R&D Systems)
95
R&D Systems methylcellulose mc stock solution
Methylcellulose Mc Stock Solution, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/methylcellulose mc stock solution/product/R&D Systems
Average 95 stars, based on 1 article reviews
methylcellulose mc stock solution - by Bioz Stars, 2026-02
95/100 stars
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macs buffer  (Miltenyi Biotec)
99
Miltenyi Biotec macs buffer
Macs Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/macs buffer/product/Miltenyi Biotec
Average 99 stars, based on 1 article reviews
macs buffer - by Bioz Stars, 2026-02
99/100 stars
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atp  (SignalChem)
93
SignalChem atp
IRE1α deficiency contributes to BMPC dysfunction, and MGO suppresses IRE1α expression at the protein level. Overexpression of IRE1α rescues BMPC dysfunction after MGO exposure. A: Western blot analysis of expression levels of IRE1α and the phosphorylated form of IRE1α (p-IRE1α) in db/db and db/+ BMPCs. Representative protein bands are shown (n = 5 per group). *P < 0.05 vs. db/+. B: Western blot analysis of IRE1⍺ level in IRE1α+/+ BMPCs infected with Ad-Cre (IRE1α−/−) or Ad-GFP (IRE1α+/+) (n = 5 per group). *P < 0.05 vs. IRE1α+/+. C: Apoptosis of IRE1Α−/− or IRE1α+/+ BMPCs was evaluated by caspase 3/7 assay (n = 5 per group). *P < 0.05 vs. IRE1α+/+. D: Network formation quantification of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). *P < 0.05 vs. IRE1α+/+. Representative pictures are shown. Scale bar = 1,000 μm. E: Proliferation of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). F: Western blot analysis of IRE1⍺ in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. G: Western blot analysis of p-IRE1 in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. H: Levels of Ire1 mRNA in db/+ BMPCs exposed to MGO (n = 5 per group). I: Western blot analysis of protein levels of PERK, ATF6, and Bip in db/+ BMPCs exposed to MGO (n = 5 per group). J: Agarose electrophoresis of IRE1α-mediated <t>RNA</t> <t>cleavage</t> assay of native and MGO-modified IRE1α. One microgram of in vitro–transcribed RNA (pre-miR-466:pre-miR-200 = 1:1) was used as RNA substrate of IRE1α. The cleavage reaction without <t>ATP</t> initiation was used as loading control. Left lane: RNA substrate without IRE1α incubation. Middle lane: spliced RNA substrate after native IRE1α incubation. Right lane: RNA substrate incubated with MGO-modified IRE1α. K: Western blot analysis of IRE1α protein expression in db/db BMPCs infected with Ad-IRE1α or Ad-GFP after exposure of 10 μmol/L MGO for 24 h (using H2O as control) (n = 5 per group). #P < 0.05 vs. db/db plus Ad-IRE1α plus MGO. L: Network formation quantification of db/db BMPCs infected with Ad-IRE1α or Ad-GFP after MGO exposure (n = 5 per group). *P < 0.05 vs. db/db plus Ad-GFP plus control; #P < 0.05 vs. db/db plus Ad-GFP plus MGO. Representative images of tube network are shown. M, protein marker. Scale bar = 1,000 μm.
Atp, supplied by SignalChem, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/atp/product/SignalChem
Average 93 stars, based on 1 article reviews
atp - by Bioz Stars, 2026-02
93/100 stars
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hsc001 methylcellulose medium  (R&D Systems)
99
R&D Systems hsc001 methylcellulose medium
IRE1α deficiency contributes to BMPC dysfunction, and MGO suppresses IRE1α expression at the protein level. Overexpression of IRE1α rescues BMPC dysfunction after MGO exposure. A: Western blot analysis of expression levels of IRE1α and the phosphorylated form of IRE1α (p-IRE1α) in db/db and db/+ BMPCs. Representative protein bands are shown (n = 5 per group). *P < 0.05 vs. db/+. B: Western blot analysis of IRE1⍺ level in IRE1α+/+ BMPCs infected with Ad-Cre (IRE1α−/−) or Ad-GFP (IRE1α+/+) (n = 5 per group). *P < 0.05 vs. IRE1α+/+. C: Apoptosis of IRE1Α−/− or IRE1α+/+ BMPCs was evaluated by caspase 3/7 assay (n = 5 per group). *P < 0.05 vs. IRE1α+/+. D: Network formation quantification of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). *P < 0.05 vs. IRE1α+/+. Representative pictures are shown. Scale bar = 1,000 μm. E: Proliferation of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). F: Western blot analysis of IRE1⍺ in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. G: Western blot analysis of p-IRE1 in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. H: Levels of Ire1 mRNA in db/+ BMPCs exposed to MGO (n = 5 per group). I: Western blot analysis of protein levels of PERK, ATF6, and Bip in db/+ BMPCs exposed to MGO (n = 5 per group). J: Agarose electrophoresis of IRE1α-mediated <t>RNA</t> <t>cleavage</t> assay of native and MGO-modified IRE1α. One microgram of in vitro–transcribed RNA (pre-miR-466:pre-miR-200 = 1:1) was used as RNA substrate of IRE1α. The cleavage reaction without <t>ATP</t> initiation was used as loading control. Left lane: RNA substrate without IRE1α incubation. Middle lane: spliced RNA substrate after native IRE1α incubation. Right lane: RNA substrate incubated with MGO-modified IRE1α. K: Western blot analysis of IRE1α protein expression in db/db BMPCs infected with Ad-IRE1α or Ad-GFP after exposure of 10 μmol/L MGO for 24 h (using H2O as control) (n = 5 per group). #P < 0.05 vs. db/db plus Ad-IRE1α plus MGO. L: Network formation quantification of db/db BMPCs infected with Ad-IRE1α or Ad-GFP after MGO exposure (n = 5 per group). *P < 0.05 vs. db/db plus Ad-GFP plus control; #P < 0.05 vs. db/db plus Ad-GFP plus MGO. Representative images of tube network are shown. M, protein marker. Scale bar = 1,000 μm.
Hsc001 Methylcellulose Medium, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hsc001 methylcellulose medium/product/R&D Systems
Average 99 stars, based on 1 article reviews
hsc001 methylcellulose medium - by Bioz Stars, 2026-02
99/100 stars
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10x mg2 atp solution  (Sino Biological)
93
Sino Biological 10x mg2 atp solution
IRE1α deficiency contributes to BMPC dysfunction, and MGO suppresses IRE1α expression at the protein level. Overexpression of IRE1α rescues BMPC dysfunction after MGO exposure. A: Western blot analysis of expression levels of IRE1α and the phosphorylated form of IRE1α (p-IRE1α) in db/db and db/+ BMPCs. Representative protein bands are shown (n = 5 per group). *P < 0.05 vs. db/+. B: Western blot analysis of IRE1⍺ level in IRE1α+/+ BMPCs infected with Ad-Cre (IRE1α−/−) or Ad-GFP (IRE1α+/+) (n = 5 per group). *P < 0.05 vs. IRE1α+/+. C: Apoptosis of IRE1Α−/− or IRE1α+/+ BMPCs was evaluated by caspase 3/7 assay (n = 5 per group). *P < 0.05 vs. IRE1α+/+. D: Network formation quantification of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). *P < 0.05 vs. IRE1α+/+. Representative pictures are shown. Scale bar = 1,000 μm. E: Proliferation of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). F: Western blot analysis of IRE1⍺ in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. G: Western blot analysis of p-IRE1 in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. H: Levels of Ire1 mRNA in db/+ BMPCs exposed to MGO (n = 5 per group). I: Western blot analysis of protein levels of PERK, ATF6, and Bip in db/+ BMPCs exposed to MGO (n = 5 per group). J: Agarose electrophoresis of IRE1α-mediated <t>RNA</t> <t>cleavage</t> assay of native and MGO-modified IRE1α. One microgram of in vitro–transcribed RNA (pre-miR-466:pre-miR-200 = 1:1) was used as RNA substrate of IRE1α. The cleavage reaction without <t>ATP</t> initiation was used as loading control. Left lane: RNA substrate without IRE1α incubation. Middle lane: spliced RNA substrate after native IRE1α incubation. Right lane: RNA substrate incubated with MGO-modified IRE1α. K: Western blot analysis of IRE1α protein expression in db/db BMPCs infected with Ad-IRE1α or Ad-GFP after exposure of 10 μmol/L MGO for 24 h (using H2O as control) (n = 5 per group). #P < 0.05 vs. db/db plus Ad-IRE1α plus MGO. L: Network formation quantification of db/db BMPCs infected with Ad-IRE1α or Ad-GFP after MGO exposure (n = 5 per group). *P < 0.05 vs. db/db plus Ad-GFP plus control; #P < 0.05 vs. db/db plus Ad-GFP plus MGO. Representative images of tube network are shown. M, protein marker. Scale bar = 1,000 μm.
10x Mg2 Atp Solution, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/10x mg2 atp solution/product/Sino Biological
Average 93 stars, based on 1 article reviews
10x mg2 atp solution - by Bioz Stars, 2026-02
93/100 stars
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5 bromo 4 chloro 3 in dolyl phosphate p toluidine salt  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology 5 bromo 4 chloro 3 in dolyl phosphate p toluidine salt
IRE1α deficiency contributes to BMPC dysfunction, and MGO suppresses IRE1α expression at the protein level. Overexpression of IRE1α rescues BMPC dysfunction after MGO exposure. A: Western blot analysis of expression levels of IRE1α and the phosphorylated form of IRE1α (p-IRE1α) in db/db and db/+ BMPCs. Representative protein bands are shown (n = 5 per group). *P < 0.05 vs. db/+. B: Western blot analysis of IRE1⍺ level in IRE1α+/+ BMPCs infected with Ad-Cre (IRE1α−/−) or Ad-GFP (IRE1α+/+) (n = 5 per group). *P < 0.05 vs. IRE1α+/+. C: Apoptosis of IRE1Α−/− or IRE1α+/+ BMPCs was evaluated by caspase 3/7 assay (n = 5 per group). *P < 0.05 vs. IRE1α+/+. D: Network formation quantification of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). *P < 0.05 vs. IRE1α+/+. Representative pictures are shown. Scale bar = 1,000 μm. E: Proliferation of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). F: Western blot analysis of IRE1⍺ in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. G: Western blot analysis of p-IRE1 in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. H: Levels of Ire1 mRNA in db/+ BMPCs exposed to MGO (n = 5 per group). I: Western blot analysis of protein levels of PERK, ATF6, and Bip in db/+ BMPCs exposed to MGO (n = 5 per group). J: Agarose electrophoresis of IRE1α-mediated <t>RNA</t> <t>cleavage</t> assay of native and MGO-modified IRE1α. One microgram of in vitro–transcribed RNA (pre-miR-466:pre-miR-200 = 1:1) was used as RNA substrate of IRE1α. The cleavage reaction without <t>ATP</t> initiation was used as loading control. Left lane: RNA substrate without IRE1α incubation. Middle lane: spliced RNA substrate after native IRE1α incubation. Right lane: RNA substrate incubated with MGO-modified IRE1α. K: Western blot analysis of IRE1α protein expression in db/db BMPCs infected with Ad-IRE1α or Ad-GFP after exposure of 10 μmol/L MGO for 24 h (using H2O as control) (n = 5 per group). #P < 0.05 vs. db/db plus Ad-IRE1α plus MGO. L: Network formation quantification of db/db BMPCs infected with Ad-IRE1α or Ad-GFP after MGO exposure (n = 5 per group). *P < 0.05 vs. db/db plus Ad-GFP plus control; #P < 0.05 vs. db/db plus Ad-GFP plus MGO. Representative images of tube network are shown. M, protein marker. Scale bar = 1,000 μm.
5 Bromo 4 Chloro 3 In Dolyl Phosphate P Toluidine Salt, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/5 bromo 4 chloro 3 in dolyl phosphate p toluidine salt/product/Santa Cruz Biotechnology
Average 92 stars, based on 1 article reviews
5 bromo 4 chloro 3 in dolyl phosphate p toluidine salt - by Bioz Stars, 2026-02
92/100 stars
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hno 3 stock solution  (Revvity)
92
Revvity hno 3 stock solution
IRE1α deficiency contributes to BMPC dysfunction, and MGO suppresses IRE1α expression at the protein level. Overexpression of IRE1α rescues BMPC dysfunction after MGO exposure. A: Western blot analysis of expression levels of IRE1α and the phosphorylated form of IRE1α (p-IRE1α) in db/db and db/+ BMPCs. Representative protein bands are shown (n = 5 per group). *P < 0.05 vs. db/+. B: Western blot analysis of IRE1⍺ level in IRE1α+/+ BMPCs infected with Ad-Cre (IRE1α−/−) or Ad-GFP (IRE1α+/+) (n = 5 per group). *P < 0.05 vs. IRE1α+/+. C: Apoptosis of IRE1Α−/− or IRE1α+/+ BMPCs was evaluated by caspase 3/7 assay (n = 5 per group). *P < 0.05 vs. IRE1α+/+. D: Network formation quantification of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). *P < 0.05 vs. IRE1α+/+. Representative pictures are shown. Scale bar = 1,000 μm. E: Proliferation of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). F: Western blot analysis of IRE1⍺ in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. G: Western blot analysis of p-IRE1 in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. H: Levels of Ire1 mRNA in db/+ BMPCs exposed to MGO (n = 5 per group). I: Western blot analysis of protein levels of PERK, ATF6, and Bip in db/+ BMPCs exposed to MGO (n = 5 per group). J: Agarose electrophoresis of IRE1α-mediated <t>RNA</t> <t>cleavage</t> assay of native and MGO-modified IRE1α. One microgram of in vitro–transcribed RNA (pre-miR-466:pre-miR-200 = 1:1) was used as RNA substrate of IRE1α. The cleavage reaction without <t>ATP</t> initiation was used as loading control. Left lane: RNA substrate without IRE1α incubation. Middle lane: spliced RNA substrate after native IRE1α incubation. Right lane: RNA substrate incubated with MGO-modified IRE1α. K: Western blot analysis of IRE1α protein expression in db/db BMPCs infected with Ad-IRE1α or Ad-GFP after exposure of 10 μmol/L MGO for 24 h (using H2O as control) (n = 5 per group). #P < 0.05 vs. db/db plus Ad-IRE1α plus MGO. L: Network formation quantification of db/db BMPCs infected with Ad-IRE1α or Ad-GFP after MGO exposure (n = 5 per group). *P < 0.05 vs. db/db plus Ad-GFP plus control; #P < 0.05 vs. db/db plus Ad-GFP plus MGO. Representative images of tube network are shown. M, protein marker. Scale bar = 1,000 μm.
Hno 3 Stock Solution, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hno 3 stock solution/product/Revvity
Average 92 stars, based on 1 article reviews
hno 3 stock solution - by Bioz Stars, 2026-02
92/100 stars
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proteinase k stock solution  (Qiagen)
96
Qiagen proteinase k stock solution
IRE1α deficiency contributes to BMPC dysfunction, and MGO suppresses IRE1α expression at the protein level. Overexpression of IRE1α rescues BMPC dysfunction after MGO exposure. A: Western blot analysis of expression levels of IRE1α and the phosphorylated form of IRE1α (p-IRE1α) in db/db and db/+ BMPCs. Representative protein bands are shown (n = 5 per group). *P < 0.05 vs. db/+. B: Western blot analysis of IRE1⍺ level in IRE1α+/+ BMPCs infected with Ad-Cre (IRE1α−/−) or Ad-GFP (IRE1α+/+) (n = 5 per group). *P < 0.05 vs. IRE1α+/+. C: Apoptosis of IRE1Α−/− or IRE1α+/+ BMPCs was evaluated by caspase 3/7 assay (n = 5 per group). *P < 0.05 vs. IRE1α+/+. D: Network formation quantification of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). *P < 0.05 vs. IRE1α+/+. Representative pictures are shown. Scale bar = 1,000 μm. E: Proliferation of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). F: Western blot analysis of IRE1⍺ in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. G: Western blot analysis of p-IRE1 in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. H: Levels of Ire1 mRNA in db/+ BMPCs exposed to MGO (n = 5 per group). I: Western blot analysis of protein levels of PERK, ATF6, and Bip in db/+ BMPCs exposed to MGO (n = 5 per group). J: Agarose electrophoresis of IRE1α-mediated <t>RNA</t> <t>cleavage</t> assay of native and MGO-modified IRE1α. One microgram of in vitro–transcribed RNA (pre-miR-466:pre-miR-200 = 1:1) was used as RNA substrate of IRE1α. The cleavage reaction without <t>ATP</t> initiation was used as loading control. Left lane: RNA substrate without IRE1α incubation. Middle lane: spliced RNA substrate after native IRE1α incubation. Right lane: RNA substrate incubated with MGO-modified IRE1α. K: Western blot analysis of IRE1α protein expression in db/db BMPCs infected with Ad-IRE1α or Ad-GFP after exposure of 10 μmol/L MGO for 24 h (using H2O as control) (n = 5 per group). #P < 0.05 vs. db/db plus Ad-IRE1α plus MGO. L: Network formation quantification of db/db BMPCs infected with Ad-IRE1α or Ad-GFP after MGO exposure (n = 5 per group). *P < 0.05 vs. db/db plus Ad-GFP plus control; #P < 0.05 vs. db/db plus Ad-GFP plus MGO. Representative images of tube network are shown. M, protein marker. Scale bar = 1,000 μm.
Proteinase K Stock Solution, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/proteinase k stock solution/product/Qiagen
Average 96 stars, based on 1 article reviews
proteinase k stock solution - by Bioz Stars, 2026-02
96/100 stars
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b3 stock solution  (Eppendorf AG)
99
Eppendorf AG b3 stock solution
IRE1α deficiency contributes to BMPC dysfunction, and MGO suppresses IRE1α expression at the protein level. Overexpression of IRE1α rescues BMPC dysfunction after MGO exposure. A: Western blot analysis of expression levels of IRE1α and the phosphorylated form of IRE1α (p-IRE1α) in db/db and db/+ BMPCs. Representative protein bands are shown (n = 5 per group). *P < 0.05 vs. db/+. B: Western blot analysis of IRE1⍺ level in IRE1α+/+ BMPCs infected with Ad-Cre (IRE1α−/−) or Ad-GFP (IRE1α+/+) (n = 5 per group). *P < 0.05 vs. IRE1α+/+. C: Apoptosis of IRE1Α−/− or IRE1α+/+ BMPCs was evaluated by caspase 3/7 assay (n = 5 per group). *P < 0.05 vs. IRE1α+/+. D: Network formation quantification of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). *P < 0.05 vs. IRE1α+/+. Representative pictures are shown. Scale bar = 1,000 μm. E: Proliferation of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). F: Western blot analysis of IRE1⍺ in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. G: Western blot analysis of p-IRE1 in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. H: Levels of Ire1 mRNA in db/+ BMPCs exposed to MGO (n = 5 per group). I: Western blot analysis of protein levels of PERK, ATF6, and Bip in db/+ BMPCs exposed to MGO (n = 5 per group). J: Agarose electrophoresis of IRE1α-mediated <t>RNA</t> <t>cleavage</t> assay of native and MGO-modified IRE1α. One microgram of in vitro–transcribed RNA (pre-miR-466:pre-miR-200 = 1:1) was used as RNA substrate of IRE1α. The cleavage reaction without <t>ATP</t> initiation was used as loading control. Left lane: RNA substrate without IRE1α incubation. Middle lane: spliced RNA substrate after native IRE1α incubation. Right lane: RNA substrate incubated with MGO-modified IRE1α. K: Western blot analysis of IRE1α protein expression in db/db BMPCs infected with Ad-IRE1α or Ad-GFP after exposure of 10 μmol/L MGO for 24 h (using H2O as control) (n = 5 per group). #P < 0.05 vs. db/db plus Ad-IRE1α plus MGO. L: Network formation quantification of db/db BMPCs infected with Ad-IRE1α or Ad-GFP after MGO exposure (n = 5 per group). *P < 0.05 vs. db/db plus Ad-GFP plus control; #P < 0.05 vs. db/db plus Ad-GFP plus MGO. Representative images of tube network are shown. M, protein marker. Scale bar = 1,000 μm.
B3 Stock Solution, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/b3 stock solution/product/Eppendorf AG
Average 99 stars, based on 1 article reviews
b3 stock solution - by Bioz Stars, 2026-02
99/100 stars
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ctni primary antibody 1ab stock solution  (HyTest)
95
HyTest ctni primary antibody 1ab stock solution
IRE1α deficiency contributes to BMPC dysfunction, and MGO suppresses IRE1α expression at the protein level. Overexpression of IRE1α rescues BMPC dysfunction after MGO exposure. A: Western blot analysis of expression levels of IRE1α and the phosphorylated form of IRE1α (p-IRE1α) in db/db and db/+ BMPCs. Representative protein bands are shown (n = 5 per group). *P < 0.05 vs. db/+. B: Western blot analysis of IRE1⍺ level in IRE1α+/+ BMPCs infected with Ad-Cre (IRE1α−/−) or Ad-GFP (IRE1α+/+) (n = 5 per group). *P < 0.05 vs. IRE1α+/+. C: Apoptosis of IRE1Α−/− or IRE1α+/+ BMPCs was evaluated by caspase 3/7 assay (n = 5 per group). *P < 0.05 vs. IRE1α+/+. D: Network formation quantification of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). *P < 0.05 vs. IRE1α+/+. Representative pictures are shown. Scale bar = 1,000 μm. E: Proliferation of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). F: Western blot analysis of IRE1⍺ in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. G: Western blot analysis of p-IRE1 in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. H: Levels of Ire1 mRNA in db/+ BMPCs exposed to MGO (n = 5 per group). I: Western blot analysis of protein levels of PERK, ATF6, and Bip in db/+ BMPCs exposed to MGO (n = 5 per group). J: Agarose electrophoresis of IRE1α-mediated <t>RNA</t> <t>cleavage</t> assay of native and MGO-modified IRE1α. One microgram of in vitro–transcribed RNA (pre-miR-466:pre-miR-200 = 1:1) was used as RNA substrate of IRE1α. The cleavage reaction without <t>ATP</t> initiation was used as loading control. Left lane: RNA substrate without IRE1α incubation. Middle lane: spliced RNA substrate after native IRE1α incubation. Right lane: RNA substrate incubated with MGO-modified IRE1α. K: Western blot analysis of IRE1α protein expression in db/db BMPCs infected with Ad-IRE1α or Ad-GFP after exposure of 10 μmol/L MGO for 24 h (using H2O as control) (n = 5 per group). #P < 0.05 vs. db/db plus Ad-IRE1α plus MGO. L: Network formation quantification of db/db BMPCs infected with Ad-IRE1α or Ad-GFP after MGO exposure (n = 5 per group). *P < 0.05 vs. db/db plus Ad-GFP plus control; #P < 0.05 vs. db/db plus Ad-GFP plus MGO. Representative images of tube network are shown. M, protein marker. Scale bar = 1,000 μm.
Ctni Primary Antibody 1ab Stock Solution, supplied by HyTest, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ctni primary antibody 1ab stock solution/product/HyTest
Average 95 stars, based on 1 article reviews
ctni primary antibody 1ab stock solution - by Bioz Stars, 2026-02
95/100 stars
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b c6f5 3 stock solution  (Eppendorf AG)
99
Eppendorf AG b c6f5 3 stock solution
IRE1α deficiency contributes to BMPC dysfunction, and MGO suppresses IRE1α expression at the protein level. Overexpression of IRE1α rescues BMPC dysfunction after MGO exposure. A: Western blot analysis of expression levels of IRE1α and the phosphorylated form of IRE1α (p-IRE1α) in db/db and db/+ BMPCs. Representative protein bands are shown (n = 5 per group). *P < 0.05 vs. db/+. B: Western blot analysis of IRE1⍺ level in IRE1α+/+ BMPCs infected with Ad-Cre (IRE1α−/−) or Ad-GFP (IRE1α+/+) (n = 5 per group). *P < 0.05 vs. IRE1α+/+. C: Apoptosis of IRE1Α−/− or IRE1α+/+ BMPCs was evaluated by caspase 3/7 assay (n = 5 per group). *P < 0.05 vs. IRE1α+/+. D: Network formation quantification of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). *P < 0.05 vs. IRE1α+/+. Representative pictures are shown. Scale bar = 1,000 μm. E: Proliferation of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). F: Western blot analysis of IRE1⍺ in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. G: Western blot analysis of p-IRE1 in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. H: Levels of Ire1 mRNA in db/+ BMPCs exposed to MGO (n = 5 per group). I: Western blot analysis of protein levels of PERK, ATF6, and Bip in db/+ BMPCs exposed to MGO (n = 5 per group). J: Agarose electrophoresis of IRE1α-mediated <t>RNA</t> <t>cleavage</t> assay of native and MGO-modified IRE1α. One microgram of in vitro–transcribed RNA (pre-miR-466:pre-miR-200 = 1:1) was used as RNA substrate of IRE1α. The cleavage reaction without <t>ATP</t> initiation was used as loading control. Left lane: RNA substrate without IRE1α incubation. Middle lane: spliced RNA substrate after native IRE1α incubation. Right lane: RNA substrate incubated with MGO-modified IRE1α. K: Western blot analysis of IRE1α protein expression in db/db BMPCs infected with Ad-IRE1α or Ad-GFP after exposure of 10 μmol/L MGO for 24 h (using H2O as control) (n = 5 per group). #P < 0.05 vs. db/db plus Ad-IRE1α plus MGO. L: Network formation quantification of db/db BMPCs infected with Ad-IRE1α or Ad-GFP after MGO exposure (n = 5 per group). *P < 0.05 vs. db/db plus Ad-GFP plus control; #P < 0.05 vs. db/db plus Ad-GFP plus MGO. Representative images of tube network are shown. M, protein marker. Scale bar = 1,000 μm.
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IRE1α deficiency contributes to BMPC dysfunction, and MGO suppresses IRE1α expression at the protein level. Overexpression of IRE1α rescues BMPC dysfunction after MGO exposure. A: Western blot analysis of expression levels of IRE1α and the phosphorylated form of IRE1α (p-IRE1α) in db/db and db/+ BMPCs. Representative protein bands are shown (n = 5 per group). *P < 0.05 vs. db/+. B: Western blot analysis of IRE1⍺ level in IRE1α+/+ BMPCs infected with Ad-Cre (IRE1α−/−) or Ad-GFP (IRE1α+/+) (n = 5 per group). *P < 0.05 vs. IRE1α+/+. C: Apoptosis of IRE1Α−/− or IRE1α+/+ BMPCs was evaluated by caspase 3/7 assay (n = 5 per group). *P < 0.05 vs. IRE1α+/+. D: Network formation quantification of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). *P < 0.05 vs. IRE1α+/+. Representative pictures are shown. Scale bar = 1,000 μm. E: Proliferation of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). F: Western blot analysis of IRE1⍺ in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. G: Western blot analysis of p-IRE1 in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. H: Levels of Ire1 mRNA in db/+ BMPCs exposed to MGO (n = 5 per group). I: Western blot analysis of protein levels of PERK, ATF6, and Bip in db/+ BMPCs exposed to MGO (n = 5 per group). J: Agarose electrophoresis of IRE1α-mediated RNA cleavage assay of native and MGO-modified IRE1α. One microgram of in vitro–transcribed RNA (pre-miR-466:pre-miR-200 = 1:1) was used as RNA substrate of IRE1α. The cleavage reaction without ATP initiation was used as loading control. Left lane: RNA substrate without IRE1α incubation. Middle lane: spliced RNA substrate after native IRE1α incubation. Right lane: RNA substrate incubated with MGO-modified IRE1α. K: Western blot analysis of IRE1α protein expression in db/db BMPCs infected with Ad-IRE1α or Ad-GFP after exposure of 10 μmol/L MGO for 24 h (using H2O as control) (n = 5 per group). #P < 0.05 vs. db/db plus Ad-IRE1α plus MGO. L: Network formation quantification of db/db BMPCs infected with Ad-IRE1α or Ad-GFP after MGO exposure (n = 5 per group). *P < 0.05 vs. db/db plus Ad-GFP plus control; #P < 0.05 vs. db/db plus Ad-GFP plus MGO. Representative images of tube network are shown. M, protein marker. Scale bar = 1,000 μm.

Journal: Diabetes

Article Title: Ameliorating Methylglyoxal-Induced Progenitor Cell Dysfunction for Tissue Repair in Diabetes

doi: 10.2337/db18-0933

Figure Lengend Snippet: IRE1α deficiency contributes to BMPC dysfunction, and MGO suppresses IRE1α expression at the protein level. Overexpression of IRE1α rescues BMPC dysfunction after MGO exposure. A: Western blot analysis of expression levels of IRE1α and the phosphorylated form of IRE1α (p-IRE1α) in db/db and db/+ BMPCs. Representative protein bands are shown (n = 5 per group). *P < 0.05 vs. db/+. B: Western blot analysis of IRE1⍺ level in IRE1α+/+ BMPCs infected with Ad-Cre (IRE1α−/−) or Ad-GFP (IRE1α+/+) (n = 5 per group). *P < 0.05 vs. IRE1α+/+. C: Apoptosis of IRE1Α−/− or IRE1α+/+ BMPCs was evaluated by caspase 3/7 assay (n = 5 per group). *P < 0.05 vs. IRE1α+/+. D: Network formation quantification of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). *P < 0.05 vs. IRE1α+/+. Representative pictures are shown. Scale bar = 1,000 μm. E: Proliferation of IRE1α−/− or IRE1α+/+ BMPCs (n = 5 per group). F: Western blot analysis of IRE1⍺ in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. G: Western blot analysis of p-IRE1 in db/+ BMPCs exposed to 10 μmol/L MGO for 24 h (n = 5 per group). *P < 0.05 vs. control. H: Levels of Ire1 mRNA in db/+ BMPCs exposed to MGO (n = 5 per group). I: Western blot analysis of protein levels of PERK, ATF6, and Bip in db/+ BMPCs exposed to MGO (n = 5 per group). J: Agarose electrophoresis of IRE1α-mediated RNA cleavage assay of native and MGO-modified IRE1α. One microgram of in vitro–transcribed RNA (pre-miR-466:pre-miR-200 = 1:1) was used as RNA substrate of IRE1α. The cleavage reaction without ATP initiation was used as loading control. Left lane: RNA substrate without IRE1α incubation. Middle lane: spliced RNA substrate after native IRE1α incubation. Right lane: RNA substrate incubated with MGO-modified IRE1α. K: Western blot analysis of IRE1α protein expression in db/db BMPCs infected with Ad-IRE1α or Ad-GFP after exposure of 10 μmol/L MGO for 24 h (using H2O as control) (n = 5 per group). #P < 0.05 vs. db/db plus Ad-IRE1α plus MGO. L: Network formation quantification of db/db BMPCs infected with Ad-IRE1α or Ad-GFP after MGO exposure (n = 5 per group). *P < 0.05 vs. db/db plus Ad-GFP plus control; #P < 0.05 vs. db/db plus Ad-GFP plus MGO. Representative images of tube network are shown. M, protein marker. Scale bar = 1,000 μm.

Article Snippet: The cleavage reactions were initiated by adding ATP (2.5 mmol/L) (A50-09-200; SignalChem).

Techniques: Expressing, Over Expression, Western Blot, Infection, Electrophoresis, Cleavage Assay, Modification, In Vitro, Incubation, Marker